Pre-Conference Focus Day
9:00 am
Registration & Morning Coffee
9:25 am Chair’s Opening Remarks
Ex Vivo Production of Large Quantities of EVs: Challenges & Opportunities
9:30 am Panel Discussion: From Lab to Patients- Biopharma View on Enhancing Scale-Up & Manufacturing Strategies of EVs
Synopsis
- Discussing advantages and limitations of EV large-scale manufacture
- Exploring enhancements of technical tools to overcome limitations
- How to maintain efficiency when moving from small-scale to large-scale EV manufacture?
10:30 am Large-Scale Manufacturing & Testing of EVs from Stem Cells & Biological Fluids in Pre- Clinical Models
Synopsis
- Exploring a bioreactor methodology for producing Mesenchymal Stem Cellderived EVs
- Harnessing biological fluids to extract therapeutic EVs
- Investigating EV products in pre-clinical cardiac and pulmonary models
11:00 am
Morning Networking Break
12:00 pm How to Successfully Translate EV Manufacture to Clinical Level
Synopsis
- Comparing different technological approaches and more of a differentiation to advance isolation and purification of EVs
- Adjusting technical tools when moving from research development to CMC
- Scaling up this manufacturing of clinical grade material in a reproducible manner
12:30 pm Scalable Mechanoregulation of EV Production & Bioactivity
Synopsis
- Using 3D printed perfusion bioreactors to increase EV production and improve EV bioactivity
- Employing microfabricated devices to exploit stem cell confinement for enhancing EV bioactivity
- Parsing roles of shear and flow in exerting effects on EV producer cells through finite control off EV producer cell microenvironment
1:00 pm
Networking Lunch
Optimized Isolation & Purification of EVs for Large-Scale Manufacturing
2:00 pm Industrially Scalable Isolation Method for Milk Derived Exosomes & Choosing Optimal Analytical Tools
Synopsis
- Is tangential flow filtration the best technology to purify exosomes?
- How isolation methods differ for specific EV populations
- Translating multi-step isolation methods to a manufacturing scale
- Learnings from the shortfalls of scaling up EVs
2:30 pm Scaling Up Stem Cell Differentiation & Purifying Exact Exosome Subpopulations
Synopsis
- How to scale up stem cell differentiation
- Unravelling analytics to characterize exact population of interest
- Purifying populations through combinations of filtration, TFL and chromatography
3:00 pm
Afternoon Networking Break
3:30 pm Extracellular Vesicles Engineering & Their Roles as Carrier of Therapeutics Agents
Synopsis
- Using EVs targeting cancer cells and human placenta which can be beneficial for women with placenta deficiencies during pregnancies
- How to scale up the production of EVs
- Scaling up a variation of different batch of EVs and certain quality control GMP conditions
- How to modify targeting of EVs
- How to incorporate molecules inside of EVs. Fusing with lipid or synthetic nanoparticles
4:00 pm Scale Up of EVs Derived from Probiotic Cells & Their Role in Immuno- Oncology
Synopsis
- Large scale production of EVs derived from attenuated bacterial cells
- How genetic engineering of these EVs leads to their non-toxic and safe use EVs
- Oncology applications and T cell stimulation in the tumour microenvironment
4:30 pm High Throughput & High Purity Exosome Isolations Using Capillary- Channeled Polymer (C-CP) Fiber Phases; From Microliters to Milliliters & Shooting for More
Synopsis
- Discussing the isolation of EVs from different cell sources e.g. blood, bacteria
- Rapid isolation and quantification of EVs for process monitoring
- High efficiency reduction of contaminants during purification for proteomic characterization
- Balancing optimal purification of extracellular vesicles with high yield in EV and therapeutic efficiency at large scale